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The practical shortcomings stem with the excessive force drop necessary to pressure cellular fluid with the column and The problem of preparing a uniform packing of really high-quality components.[24] Each time particle size is reduced appreciably, An additional spherical of instrument advancement commonly will have to occur to deal with the strain
The main parts of the HPLC are shown in Figure (PageIndex 3 ). The position of the pump is to force a liquid (cellular period) as a result of at a specific movement fee (milliliters for every moment). The injector serves to introduce the liquid sample into the flow stream in the cellular period. Column is the most central and crucial element of HPL
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